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r d systems cat af875  (R&D Systems)


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    Structured Review

    R&D Systems r d systems cat af875
    R D Systems Cat Af875, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/r+d+systems+cat+af875/Human+IGFBP-5+Antibody/pmc08795238__41698_2022_251_MOESM1_ESM-19-40-40
    Average 93 stars, based on 18 article reviews
    r d systems cat af875 - by Bioz Stars, 2026-11
    93/100 stars

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    Blocking Assay:

    Article Title: Single-cell analysis of gastric pre-cancerous and cancer lesions reveals cell lineage diversity and intratumoral heterogeneity
    Article Snippet: .. The slides were washed again with PBS, blocked for 2 h at room temperature with SerumFree Protein Block (Dako, Cat# X0909), probed at 4°C overnight with the primary antibodies at 1/1000 dilution (anti-SRF, Cell signaling Tech, Cat# D71A9; anti-IGFBP5, R&D systems, Cat# AF875; antiMRTFA, Abcam, Cat# ab49311), stained for 30 min with horseradish peroxidase (HRP)-conjugated anti-rabbit IgG (Dako, Cat# K4003) or Goat-IgG VECTASTAIN ABC-HRP Kit (Vector Cat# PK-4005), and developed with Liquid DAB+ Substrate Chromogen System (Dako, Cat# K3468). .. Finally, the specimens were counterstained with Mayer’s hematoxylin (Dako, Cat# S3309) and mounted with Shandon Synthetic Mount (Thermo Cat# 6769007).

    Article Title: Single-cell analysis of gastric pre-cancerous and cancer lesions reveals cell lineage diversity and intratumoral heterogeneity
    Article Snippet: .. The slides were washed again with PBS, blocked for 2 h at room temperature with Serum- Free Protein Block (Dako, Cat# X0909), probed at 4°C overnight with the primary antibodies at 1/1000 dilution (anti-SRF, Cell signaling Tech, Cat# D71A9; anti-IGFBP5, R&D systems, Cat# AF875; anti- MRTFA, Abcam, Cat# ab49311), stained for 30 min with horseradish peroxidase (HRP)-conjugated anti-rabbit IgG (Dako, Cat# K4003) or Goat-IgG VECTASTAIN ABC-HRP Kit (Vector Cat# PK-4005), and developed with Liquid DAB+ Substrate Chromogen System (Dako, Cat# K3468). .. Finally, the specimens were counterstained with Mayer’s hematoxylin (Dako, Cat# S3309) and mounted with Shandon Synthetic Mount (Thermo Cat# 6769007).

    Staining:

    Article Title: Single-cell analysis of gastric pre-cancerous and cancer lesions reveals cell lineage diversity and intratumoral heterogeneity
    Article Snippet: .. The slides were washed again with PBS, blocked for 2 h at room temperature with SerumFree Protein Block (Dako, Cat# X0909), probed at 4°C overnight with the primary antibodies at 1/1000 dilution (anti-SRF, Cell signaling Tech, Cat# D71A9; anti-IGFBP5, R&D systems, Cat# AF875; antiMRTFA, Abcam, Cat# ab49311), stained for 30 min with horseradish peroxidase (HRP)-conjugated anti-rabbit IgG (Dako, Cat# K4003) or Goat-IgG VECTASTAIN ABC-HRP Kit (Vector Cat# PK-4005), and developed with Liquid DAB+ Substrate Chromogen System (Dako, Cat# K3468). .. Finally, the specimens were counterstained with Mayer’s hematoxylin (Dako, Cat# S3309) and mounted with Shandon Synthetic Mount (Thermo Cat# 6769007).

    Article Title: Single-cell analysis of gastric pre-cancerous and cancer lesions reveals cell lineage diversity and intratumoral heterogeneity
    Article Snippet: .. The slides were washed again with PBS, blocked for 2 h at room temperature with Serum- Free Protein Block (Dako, Cat# X0909), probed at 4°C overnight with the primary antibodies at 1/1000 dilution (anti-SRF, Cell signaling Tech, Cat# D71A9; anti-IGFBP5, R&D systems, Cat# AF875; anti- MRTFA, Abcam, Cat# ab49311), stained for 30 min with horseradish peroxidase (HRP)-conjugated anti-rabbit IgG (Dako, Cat# K4003) or Goat-IgG VECTASTAIN ABC-HRP Kit (Vector Cat# PK-4005), and developed with Liquid DAB+ Substrate Chromogen System (Dako, Cat# K3468). .. Finally, the specimens were counterstained with Mayer’s hematoxylin (Dako, Cat# S3309) and mounted with Shandon Synthetic Mount (Thermo Cat# 6769007).



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    93
    R&D Systems r d systems cat af875
    R D Systems Cat Af875, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/r+d+systems+cat+af875/Human+IGFBP-5+Antibody/pmc08795238__41698_2022_251_MOESM1_ESM-19-40-40
    Average 93 stars, based on 1 article reviews
    r d systems cat af875 - by Bioz Stars, 2026-11
    93/100 stars
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